Studies on the Lactose Transport System in Escherichia coli

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Studies on the Lactose Transport System in Escherichia coli

I should like to discuss very briefly the current status of studies in our laboratory of the membrane protein component of the lactose transport system of Escherichia coll. Previous publications (I, 2) have presented evidence that this protein, which can be selectively labeled with radioactive N-ethylmaleimide, is the product of the y gene of the lac operon, the long-sought permease protein. Th...

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A mutant (ML 308-22) was isolated from Escherichia coli ML 308, which had lost the normal capacity to accumulate lactose analogs despite an increase in the membrane carrier activity. The exit of thiomethylgalactoside was much faster than normal, accounting for the inability of the cell to maintain high intracellular concentrations of galactosides. Growth of the mutant on lactose was normal at h...

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The active accumulation of maltose and maltodextrins by Escherichia coli is dependent on the maltose transport system. Several lines of evidence suggest that the substrate specificity of the system is not only determined by the periplasmic maltose-binding protein but that a further level of substrate specificity is contributed by the inner membrane integral membrane components of the system, Ma...

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Lactose permeation via the arabinose transport system in Escherichia coli K-12.

This paper describes the isolation and characterization of a mutant of Escherichia coli that transports lactose and its analog thiomethylgalactoside via the arabinose permeation system. Unlike transport via the lactose permease, this transport is not inhibited by thiodigalactoside, but was inhibited by arabinose, xylose, and fucose. The site of the mutation was in the arabinose C gene and confe...

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GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.

The brilliant contributions of workers at the Institut Pasteur have revealed the existence of a specific, genetically controlled transport system that mediates the uptake of lactose in Escherichia coli. Work on this transport system has been the subject of numerous reports and reviews.--' We have recently reported evidence that a membrane-bound protein distinct from 0-galactosidase and thiogala...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1969

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.54.1.91